Molecular detection of Candida species from hospitalized patients’ specimens

Objective To identify the most frequent Candida species in specimens from patients hospitalized different medical centers of Mexico City, with suspected fungal infection. Methods Specimens were grown on Sabouraud dextrose agar at 28°C for 72 h. In addition, DNA was extracted. Isolates CHROMagar Candida™, 37°C 48 The molecular identification performed by polymerase chain reaction (PCR) using primers specific four species. Results Eighty one processed and included: bronchial lavage, pleural, cerebrospinal, peritoneal, ascites bile fluids; blood, sputum, bone marrow, oro-tracheal cannula ganglion. By culture, 30 samples (37%) positive, PCR, 41 (50.6%). frequency was: albicans 82.9%, tropicalis 31.7%, glabrata 24.4%, parapsilosis 4.9%. 34.1% a mixture detected suggesting co-infection: Two five (C. albicans-C C. glabrata), three albicans-C. glabrata-C. tropicalis). Conclusions PCR is an useful tool detection common causing infection patients, it avoids requirement culture weather we start clinical specimen favors early diagnosis invasive candidiasis.